A link between arabinose utilization and oxalotrophy in Bradyrhizobium japonicum.

نویسندگان

  • Marion Koch
  • Nathanaël Delmotte
  • Christian H Ahrens
  • Ulrich Omasits
  • Kathrin Schneider
  • Francesco Danza
  • Barnali Padhi
  • Valérie Murset
  • Olivier Braissant
  • Julia A Vorholt
  • Hauke Hennecke
  • Gabriella Pessi
چکیده

Rhizobia have a versatile catabolism that allows them to compete successfully with other microorganisms for nutrients in the soil and in the rhizosphere of their respective host plants. In this study, Bradyrhizobium japonicum USDA 110 was found to be able to utilize oxalate as the sole carbon source. A proteome analysis of cells grown in minimal medium containing arabinose suggested that oxalate oxidation extends the arabinose degradation branch via glycolaldehyde. A mutant of the key pathway genes oxc (for oxalyl-coenzyme A decarboxylase) and frc (for formyl-coenzyme A transferase) was constructed and shown to be (i) impaired in growth on arabinose and (ii) unable to grow on oxalate. Oxalate was detected in roots and, at elevated levels, in root nodules of four different B. japonicum host plants. Mixed-inoculation experiments with wild-type and oxc-frc mutant cells revealed that oxalotrophy might be a beneficial trait of B. japonicum at some stage during legume root nodule colonization.

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منابع مشابه

Synthesis of Exopolysaccharide by Bradyrhizobium japonicum during Growth on Hydroaromatic Substrates.

The hydroaromatic acids shikimate and quinate, which may be available as carbon sources in the soil, supported production of only low levels of acidic exopolysaccharide by Bradyrhizobium japonicum. Exopolysaccharide production (micrograms per 10 cells) was 4.9 on quinate and 4.5 on shikimate; in comparison, it was 128 on adipate, 18 on l-arabinose, and 39 on d-glucose.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 80 7  شماره 

صفحات  -

تاریخ انتشار 2014